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Scaling-up of high-cell-density fermentation (HCDF) of Pichia pastoris from the lab or pilot scale to the demonstration scale possesses great significance because the latter is the final technological hurdle in the decision to go commercial. Drmdbg And Drm2wmv. However, related investigations have rarely been reported. In this paper, we study the scaling-up processes of a recombinant P. Actual Installer 4 2 Serial Number.

Pastoris from the pilot (10 to 100-L) to the demonstration (1,000-L) scales, which can be used to convert 7- β-xylosyl-10-deacetyltaxol into 10-deacetyltaxol by the β-xylosidase for semi-synthesis of Taxol. We demonstrated that a pure oxygen supplement can be omitted from the HCDF if the super atmospheric pressure was increased from 0.05 to 0.10 ± 0.05 MPa, and we developed a new methanol feeding biomass -stat strategy (0. Boss Amp 250n Manual. 035 mL/g/h) with 1% dissolved oxygen and 100 g/L initial induction biomass (dry cell weight).